With the development of global pandemic, viral nuclei acid detection has demonstrated its importance again in monkeypox prevention and diagnostics. However, the accuracy and consistency of large-amount assays still need to be further validated. GeneWell monkeypox virus reference standards set contains multiple target sequences of Monkeypox, cowpox, and smallpox virus in pseudoviral solution format. It can be used to assess the performance, proficiency, consistency and accuracy of the whole workflow of nucleic acid detection tests. Cowpox and smallpox virus reference standards are also compatible as negative controls to evaluate the specification of testing process.