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Onco SNV Multiplex ctDNA Reference Standard Set

Product Code Specification
IB-GW-OCTM009 20ng/μL, 0.5μg/tube
  • Intro
Onco SNV multiplex ctDNA reference standard set is derived from human cell lines and covers 8 cancer-related variants in gradient allelic frequencies (0.1%, 1%) and matched wildtype (0%). These allelic frequencies are all precisely verified by ddPCR to contribute to the LoD determination and performance validation. Furthermore, it can also be applied for sensitivity verification, assay development and routine quality controls for both NGS and PCR-based assays. With included widetype reference standard, the onco SNV multiplex ctDNA set can also help to challenge those false negative results.
  • Product information
  • Application fields
  • Resources
Product Format ctDNA
Fragment Size ~160bp
Description Three tubes set containing the same variants with 0.1%, 1%, and 0% AFs
Buffer Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Specification 20ng/μL, 0.5μg/tube*3
Storage Condition 2-8℃
Shelf Life 36 months
Test Test Method Acceptance Criteria
Allele Frequency (AF) and Genotype Droplet Digital PCR AF = 0%, accepted range = ≤0.1%; AF ＜1%, accepted range = ± 50% (0.1% AF = ≥0.05% + wildtype ); 1% ≤AF ＜ 5%, accepted range =± 40%; 5% ≤AF ＜ 10%, accepted range = ± 30%; 10% ≤AF, accepted range = ± 20%; Copy Number＜ 5, accepted range = ± 40%; 5 ≤ Copy Number ＜10, accepted range = ± 30%
Fragmentation Size D1000 DNA ScreenTape assay 160bp±10%
Quantification Qubit dsDNA BR assay 17-23 ng/μl
Number Variant Type Mutation CDS Mutation Expected Allelic Frequency(%)/Copy Number COSMIC ID Chromosome Number Genomosome Position
1 SNV EGFR L858R c.2573T＞G 0%，0.1%，1% COSM6224 7 55191822
2 SNV EGFR T790M c.2369C＞T 0%，0.1%，1% COSM6240 7 55181373
3 Insertion EGFR V769_D770insASV c.2307_2308insGCCAGCGTG 0%，0.1%，1% COSM12376 7 55181317-55181318
4 Deletion EGFR ΔE746_A750 c.2235_2249del15 0%，0.1%，1% COSM6223 7 55174772-55174786
5 SNV KRAS A146T c.436G＞A 0%，0.1%，1% COSM19404 12 25225628
6 SNV KRAS G12D c.35G＞A 0%，0.1%，1% COSM521 12 25245350
7 SNV NRAS Q61K c.181C＞A 0%，0.1%，1% COSM580 1 114713909
8 SNV PIK3CA E545K c.1633G＞A 0%，0.1%，1% COSM763 3 179218303
  • Assay accuracy
  • Repeatability
  • Robustness
  • LOD determination
  • Workflow perfomance
  • Cancer panel assessment
  • Platform comparison
IB-GW-OCTM009-Package Insert： download
ctDNA-Flyer： download
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