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PancancerLight 800 gDNA Reference Standard

Product Code Specification
IB-GW-OGTM800 30ng/µL,1µg/tube
  • Intro
PancancerLight 800 gDNA Reference Standard is a well-characterised, cell line-derived Reference Standard containing more than 720 variants across 330 key cancer genes. This includes 450 variants with a COSMIC ID. Variants are present between 1-100% allelic frequency (AF), with 52 variants present at ≤ 20% AF for LOD determination of your assay. Every batch of PancancerLight 800 gDNA has 16 variants confirmed by ddPCR, in addition to being fully exome sequenced at 500x coverage using Illumina sequencing.
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  • Application fields
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Buffer Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Specification 30ng/µL, 1µg/tube
Storage Condition 2-8˚C
Shelf Life 36 months
Test Test Method Acceptance Criteria
Allele Frequency (AF) and Genotype Droplet Digital PCR AF = 0%, accepted range =≤0.1%; AF ＜5%, accepted range =± 30%; 5% ≤AF ＜ 10%, accepted range = ± 20%; 10% ≤AF, accepted range = ± 10%; Copy Number＜ 5, accepted range =± 30%; 5 ≤ Copy Number ＜10, accepted range = ± 20%; 10 ≤ Copy Number, accepted range = ± 10%
Quality Agarose gel electrophoresis Bright band of high molecular weight
Quantification Qubit? dsDNA BR Assay 30 ng/μL±10%
Number Variant Type Mutation CDS Mutation Expected Allelic Frequency(%)/Copy Number COSMIC ID Chromosome Number Genomosome Position
1 SNV EGFR L858R 2573T＞G 1% COSM6224 7 55191822
2 SNV KRAS A146T c.436G＞A 1% COSM19404 12 25225628
3 SNV NRAS Q61K c.181C＞A 1% COSM580 1 114713909
4 SNV EGFR T790M 2369C＞T 2% COSM6240 7 55187178
5 Deletion EGFR ΔE746-A750 c.2235_2249del15 2% COSM6223 7 55174772-55174786
6 Deletion FLT3 ΔI836 2506_2508delATC 2% COSM797 13 28018500-28018502
7 SNV KIT D816V 2447A＞T 2% COSM1314 4 54733155
8 SNV KRAS G12D c.35G＞A 2% COSM1314 4 54733155
9 Insertion EGFR V769_D770 insASV c.2307_2308insGCCAGCGTG 3% COSM12376 7 55181317-55181318
10 SNV EGFR G719S c.2155G＞A 4% COSM6252 7 55174014
11 SNV KRAS G13D c.38G＞A 4% COSM532 12 25245347
12 Fusion EML4-ALK Fusion ML4:r.1_929_ALK:r.4080_6220 EML4{ENST00000318522} ; ALK{ENST00000389048} 5% COSF734 2,2 42472827,29446394
13 Fusion CD74-ROS1 Fusion CD74:r.1_627_ROS1:r.5757_7368 CD74{ENST00000009530} ; ROS1{NM_002944} 6% COSF1201 6,5 117700222,149782188
14 SNV BRAF V600E c.1799T＞A 7% COSM476 7 140753336
15 SNV PIK3CA H1047R c.3140A＞G 7% COSM775 7 140753336
16 CNV ERBB2 Amplification NA 5copies NA 17 39687914-39730426
  • Tumor Companion Diagnostic Panel: Accuracy Validation, Repeatability Detection, Robust Validation, Limits of detection (LODs) , Comparison of Genotype Detecting Perfomance
  • Comparison of WES Process
  • Consistency Comparison of WES and Panel
IB-GW-OGTM800-Package Insert： download
IB-GW-OGTM800-Flyer： download
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