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Onco SNV Multiplex 1-25% gDNA Reference Standard

CatalogNo. specification inventory
GW-OGTM004 1µg/Tube 9999
  • Intro
Next generation sequencing (NGS) platforms give researchers much greater power when profiling the complex human genome. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers.      
Genewell’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels.     
Onco SNV Multiplex 1-25% gDNA  Reference Standard is a highly characterized, biologically relevant quality control materials. It covers 9 SNV mutations and 1 deletion.     
With this product you are able to analyze the sensitivity and specificity of your assay,  optimize and validate new cancer panels and routinely monitor the performance of your assay.    
  • Product information
  • Application fields
  • File download
浓度 50ng/ul
保存溶剂 Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
存储温度 2-8˚C
保质期 36 months
规格 1µg/tube

Authenticated Mutation

Test Test Method Acceptance Criteria
Allele Frequency (AF) and Genotype Droplet Digital PCR AF = 0%, accepted range =≤0.1%; AF ＜5%, accepted range =± 30%; 5% ≤AF ＜ 10%, accepted range = ± 20%; 10% ≤AF, accepted range = ± 10%; Copy Number＜ 5, accepted range =± 30%; 5 ≤ Copy Number ＜10, accepted range = ± 20%; 10 ≤ Copy Number, accepted range = ± 10%
Quality Agarose gel electrophoresis Bright band of high molecular weight
Quantification Spectrophotometry (A260) 45-55 ng/μl
Number Variant Type Mutation CDS Mutation Expected Allelic Frequency(%)/Copy Number COSMIC ID Chromosome Number Genomosome Position
1 SNV BRAF V600E c.1799T＞A 10.5% COSM476 7 140753336
2 SNV EGFR G719S c.2155G＞A 24.5% COSM6252 7 55174014
3 SNV EGFR L858R c.2573T＞G 3.0% COSM6224 7 55191822
4 SNV EGFR T790M c.2369C＞T 1.0% COSM6240 7 55181378
5 Deletion EGFR ΔE746_A750 c.2235_2249del15 2.0% COSM6223 7 55174772-55174786
6 SNV KRAS G12D c.35G＞A 6.0% COSM521 12 25245350
7 SNV KRAS G13D c.38G＞A 15.0% COSM532 12 25245347
8 SNV NRAS Q61K c.181C＞A 12.5% COSM580 1 114713909
9 SNV PIK3CA E545K c.1633G＞A 9.0% COSM763 3 179218303
10 SNV PIK3CA H1047R c.3140A＞G 17.5% COSM775 3 179234297
  • Assess the performance of detection workflow
  • Assess the backgroud parameter effect with the testing
  • Validate the robustness of the bioinformatics process
  • Optimize and validate new cancer panels and routinely monitor the performance of your assay
  • Gain certainty of the limit of detection and limit of quantification
  • Analyze the sensitivity, accuracy, and specificity of your assay
  • Compare platform variability
GW-OGTM004产品说明书： download
GW-OGTM004产品说明书附件-201810005： download
COA-GW-OGTM004_201810005： download
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