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Onco SNV Multiplex 5% gDNA Reference Standard II

CatalogNo. specification inventory
IB-GW-OGTM006 1µg/Tube 9999
  • Intro
Next generation sequencing (NGS) platforms give researchers much greater power when profiling the complex human genome. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers.      
Genewell’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels.     
Onco SNV Multiplex 5% gDNA  Reference Standard is a highly characterized, biologically relevant quality control materials. It covers 10 SNV mutations and 1 deletion.     
With this product you are able to analyze the sensitivity and specificity of your assay,  optimize and validate new cancer panels and routinely monitor the performance of your assay.
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浓度 30ng/µL
保存溶剂 Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
存储温度 2-8˚C
保质期 36 months
规格 1µg/tube

Authenticated Mutation

Test Test Method Acceptance Criteria
Allele Frequency (AF) and Genotype Droplet Digital PCR AF = 0%, accepted range =≤0.1%; AF ＜5%, accepted range =± 30%; 5% ≤AF ＜ 10%, accepted range = ± 20%; 10% ≤AF, accepted range = ± 10%; Copy Number＜ 5, accepted range =± 30%; 5 ≤ Copy Number ＜10, accepted range = ± 20%; 10 ≤ Copy Number, accepted range = ± 10%
Quality Agarose gel electrophoresi Bright band of high molecular weight
Quantification Qubit? dsDNA BR Assay 30 ng/μL±10%
Number Variant Type Mutation CDS Mutation Expected Allelic Frequency(%)/Copy Number COSMIC ID Chromosome Number Genomosome Position
1 SNV BRAF V600E c.1799T＞A 8% COSM476 7 140753336
2 SNV EGFR G719S c.2155G＞A 5% COSM6252 7 55174014
3 SNV EGFR L858R c.2573T＞G 5% COSM6224 7 55191822
4 SNV EGFR T790M c.2369C＞T 5% COSM6240 7 55181378
5 Deletion EGFR ΔE746_A750 c.2235_2249del15 5% COSM6223 7 55174772-55174786
6 SNV EGFR V769_D770insASV c.2307_2308insGCCAGCGTG 5% COSM12376 7 55181317-55181318
7 SNV KRAS G12C c.35G＞T 5% COSM516 12 25245351
8 SNV KRAS G13D c.38G＞A 5% COSM532 12 25245347
9 SNV NRAS Q61R c.181C＞A 5% COSM584 1 114713908
10 SNV PIK3CA E545K c.1633G＞A 5% COSM763 3 179218303
11 SNV PIK3CA H1047R c.3140A＞G 5% COSM775 3 179234297
12 Skipping MET Exon 14 Skipping c.2942_3082del 5% COSM24687 7 116771851.116774882
13 Insertion Her2 A775_G776insYVMA c.2313_2324dup 5% COSM20959 17 39724742..39724743
14 SNV FGFR3 Y375C c.G1124A 5% COSM718 4 1804372
  • Assess the performance of detection workflow
  • Assess the backgroud parameter effect with the testing
  • Validate the robustness of the bioinformatics process
  • Optimize and validate new cancer panels and routinely monitor the performance of your assay
  • Gain certainty of the limit of detection and limit of quantification
  • Analyze the sensitivity, accuracy, and specificity of your assay
  • Compare platform variability
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