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Onco SNV Multiplex 5% gDNA Reference StandardⅡ

Product Code Specification
IB-GW-OGTM006 30ng/µL, 1µg/Tube
  • Intro
Onco SNV multiplex 5% gDNA Reference Standard is a cell line derived and highly characterised quality control material, which maximally mimics clinical sample to provide a convenience approach from panel development to routine quality control. It covers 11 mutations at 5% allelic frequency,verified by droplet digital PCR (ddPCR). With this reference standard, you are able to analysis the sensitivity and specificity, optimize and validate new cancer panels and routinely monitor the performance of your assay.
  • Product information
  • Application fields
  • Resources
Related Diseases NSCLC，breast cancers，colorectal cancer, etc.
Buffer Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Specification 30ng/µL, 1µg/tube
Storage Condition 2-8˚C
Shelf Life 36 months
Test Test Method Acceptance Criteria
Allele Frequency (AF) and Genotype Droplet Digital PCR AF = 0%, accepted range =≤0.1%; AF ＜5%, accepted range =± 30%; 5% ≤AF ＜ 10%, accepted range = ± 20%; 10% ≤AF, accepted range = ± 10%; Copy Number＜ 5, accepted range =± 30%; 5 ≤ Copy Number ＜10, accepted range = ± 20%; 10 ≤ Copy Number, accepted range = ± 10%
Quality Agarose gel electrophoresi Bright band of high molecular weight
Quantification Qubit? dsDNA BR Assay 30 ng/μL±10%
Number Variant Type Mutation CDS Mutation Expected Allelic Frequency(%)/Copy Number COSMIC ID Chromosome Number Genomosome Position
1 SNV BRAF V600E c.1799T＞A 8% COSM476 7 140753336
2 SNV EGFR G719S c.2155G＞A 5% COSM6252 7 55174014
3 SNV EGFR L858R c.2573T＞G 5% COSM6224 7 55191822
4 SNV EGFR T790M c.2369C＞T 5% COSM6240 7 55181378
5 Deletion EGFR ΔE746_A750 c.2235_2249del15 5% COSM6223 7 55174772-55174786
6 SNV EGFR V769_D770insASV c.2307_2308insGCCAGCGTG 5% COSM12376 7 55181317-55181318
7 SNV KRAS G12C c.35G＞T 5% COSM516 12 25245351
8 SNV KRAS G13D c.38G＞A 5% COSM532 12 25245347
9 SNV NRAS Q61R c.181C＞A 5% COSM584 1 114713908
10 SNV PIK3CA E545K c.1633G＞A 5% COSM763 3 179218303
11 SNV PIK3CA H1047R c.3140A＞G 5% COSM775 3 179234297
12 Skipping MET Exon 14 Skipping c.2942_3082del 5% COSM24687 7 116771851.116774882
13 Insertion Her2 A775_G776insYVMA c.2313_2324dup 5% COSM20959 17 39724742..39724743
14 SNV FGFR3 Y375C c.G1124A 5% COSM718 4 1804372
  • Assess the performance of detection workflow
  • Assess the backgroud parameter effect with the testing
  • Validate the robustness of the bioinformatics process
  • Optimize and validate new cancer panels and routinely monitor the performance of your assay
  • Gain certainty of the limit of detection and limit of quantification
  • Analyze the sensitivity, accuracy, and specificity of your assay
  • Compare platform variability
IB-GW-OGTM006- Package Insert： download
IB-GW-OGTM006-Flyer： download
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