5% EGFR V769_D770insASV gDNA Reference Standard
CatalogNo. | specification |
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IB-GW-OGTS152 | 1µg/Tube |
CatalogNo. | specification |
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IB-GW-OGTS152 | 1µg/Tube |
保存溶剂 | Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0 |
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存储温度 | 2-8˚C |
保质期 | 36 months |
浓度 | 50ng/µl |
Test | Test Method | Acceptance Criteria |
---|---|---|
Allele Frequency (AF) and Genotype | Droplet Digital PCR | AF = 0%, accepted range =≤0.1%; AF <5%, accepted range =± 30%; 5% ≤AF < 10%, accepted range = ± 20%; 10% ≤AF, accepted range = ± 10%; Copy Number< 5, accepted range =± 30%; 5 ≤ Copy Number <10, accepted range = ± 20%; 10 ≤ Copy Number, accepted range = ± 10% |
Quality | Agarose gel electrophoresis | Bright band of high molecular weight |
Quantification | Spectrophotometry (A260) | 45-55 ng/μl |
Variant Type | Mutation | CDS Mutation | Expected Allelic Frequency(%)/Copy Number | COSMIC ID | Chromosome Number | Genomosome Position |
---|---|---|---|---|---|---|
Insertion | EGFR V769_D770insASV | c.2307_2308insGCCAGCGTG | 5% | COSM12376 | 7 | 55181317-55181318 |
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