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Onco SNV 1-25% gDNA Reference Standard

Product Code Specification
IB-GW-OGTM004 30ng/µL, 1µg/Tube
  • Intro
Onco SNV multiplex 1-25% gDNA reference Standard is a highly characterised and biologically relevant quality control material, covering 10 mutation at 1-25% gradient allelic frequencies. It comes in purified genomic DNA solution format and is ready to use in any NGS-based applications including whole-genome, whole-exome, custom capture and targeted amplicon panels.

With this reference standard, you are able to analysis the sensitivity and specificity, optimize and validate new cancer panels and routinely monitor the performance of your assay.
Additionally, comparing with Onco SNV multiplex 5% gDNA Reference Standard, it can further contribute to the validation of assay performance in various levels of allelic frequencies.
  • Product information
  • Application fields
  • Resources
Related Diseases NSCLC，breast cancers，colorectal cancer, etc.
Buffer Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Specification 30ng/µL, 1µg/tube
Storage Condition 2-8˚C
Shelf Life 36 months
Test Test Method Acceptance Criteria
Allele Frequency (AF) and Genotype Droplet Digital PCR AF = 0%, accepted range =≤0.1%; AF ＜5%, accepted range =± 30%; 5% ≤AF ＜ 10%, accepted range = ± 20%; 10% ≤AF, accepted range = ± 10%; Copy Number＜ 5, accepted range =± 30%; 5 ≤ Copy Number ＜10, accepted range = ± 20%; 10 ≤ Copy Number, accepted range = ± 10%
Quality Agarose gel electrophoresis Bright band of high molecular weight
Quantification Spectrophotometry (A260) 45-55 ng/μl
Number Variant Type Mutation CDS Mutation Expected Allelic Frequency(%)/Copy Number COSMIC ID Chromosome Number Genomosome Position
1 SNV BRAF V600E c.1799T＞A 10.5% COSM476 7 140753336
2 SNV EGFR G719S c.2155G＞A 24.5% COSM6252 7 55174014
3 SNV EGFR L858R c.2573T＞G 3.0% COSM6224 7 55191822
4 SNV EGFR T790M c.2369C＞T 1.0% COSM6240 7 55181378
5 Deletion EGFR ΔE746_A750 c.2235_2249del15 2.0% COSM6223 7 55174772-55174786
6 SNV KRAS G12D c.35G＞A 6.0% COSM521 12 25245350
7 SNV KRAS G13D c.38G＞A 15.0% COSM532 12 25245347
8 SNV NRAS Q61K c.181C＞A 12.5% COSM580 1 114713909
9 SNV PIK3CA E545K c.1633G＞A 9.0% COSM763 3 179218303
10 SNV PIK3CA H1047R c.3140A＞G 17.5% COSM775 3 179234297
  • Assess the performance of detection workflow
  • Assess the backgroud parameter effect with the testing
  • Validate the robustness of the bioinformatics process
  • Optimize and validate new cancer panels and routinely monitor the performance of your assay
  • Gain certainty of the limit of detection and limit of quantification
  • Analyze the sensitivity, accuracy, and specificity of your assay
  • Compare platform variability
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