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Onco Wildtype gDNA Reference Standard

Product Code Specification
IB-GW-OGTM005 30ng/µL, 5µg/Tube
  • Intro
Onco wildtype gDNA reference standard is a patient sample mimicking quality control material that verified to be wildtype in over 14 cancer-related hotspot variants. It can be used as a negative control for platform verification and run together with other GeneWell gDNA reference standards as a genomic matched wildtype.
  • Product information
  • Application fields
  • Resources
Related Diseases NSCLC，breast cancers，colorectal cancer, etc.
Buffer Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Specification 30ng/µL,5µg/tube
Storage Condition 2-8˚C
Shelf Life 36 months
Test Test Method Acceptance Criteria
Allele Frequency (AF) and Genotype Droplet Digital PCR AF = 0%, accepted range =≤0.1%; AF ＜5%, accepted range =± 30%; 5% ≤AF ＜ 10%, accepted range = ± 20%; 10% ≤AF, accepted range = ± 10%; Copy Number＜ 5, accepted range =± 30%; 5 ≤ Copy Number ＜10, accepted range = ± 20%; 10 ≤ Copy Number, accepted range = ± 10%
Quality Agarose gel electrophoresis Bright band of high molecular weight
Quantification Qubit? dsDNA BR Assay 30 ng/μL±10%
Number Variant Type Mutation CDS Mutation Expected Allelic Frequency(%)/Copy Number COSMIC ID Chromosome Number Genomosome Position
1 SNV AKT1 E17K c.49G＞A 0% COSM33765 14 104780214
2 SNV BRAF V600E c.1799T＞A 0% COSM476 7 140753336
3 SNV EGFR G719S c.2155G＞A 0% COSM6252 7 55174014
4 SNV EGFR L858R c.2573T＞G 0% COSM6224 7 55191822
5 SNV EGFR T790M c.2369C＞T 0% COSM6240 7 55181378
6 Insertion EGFR V769_D770insASV c.2307_2308insGCCAGCGTG 0% COSM12376 7 55181317-55181318
7 Deletion EGFR ΔE746_A750 c.2235_2249del15 0% COSM6223 7 55174772-55174786
8 Deletion FLT3 ΔI836 c.2506_2508delATC 0% COSM797 13 28018500-28018502
9 SNV KIT D816V c.2447A＞T 0% COSM1314 4 54733155
10 ANV KRAS A146T c.436G＞A 0% COSM19404 12 25225628
11 SNV KRAS G12D c.35G＞A 0% COSM521 12 25245350
12 SNV KRAS G13D c.38G＞A 0% COSM532 12 25245347
13 SNV NRAS Q61K c.181C＞A 0% COSM580 1 114713909
14 SNV PIK3CA E545K c.1633G＞A 0% COSM763 3 179218303
15 SNV PIK3CA H1047R c.3140A＞G 0% COSM775 3 179234297
16 Fusion CD74-ROS1 Fusion NA 0% COSF1200 6,5 117324415,150404680
17 Fusion EML4-ALK Fusion NA 0% COSF474 2,2 42264951,29223528
18 CNV ERBB2 Amplification NA 2 copies NA 17 39687914-39730426
19 CNV MET Amplification NA 2 copies NA 7 116672196-116798386
20 Fusion EML4-ALK Fusion V1 NA 0% COSF408 2,2 4229551629223520
21 SNV KRAS G12C c.34G>T 0% COSM516 12 25245351
22 SNV KRAS G12V c.35G>T 0% COSM520 12 25245350
23 SNV EGFR L861Q c.2582T>A 0% COSM6213 7 55191831
24 SNV EGFR S768I c.2303G>T 0% COSM6241 7 55181312
25 SNV KRAS G12S c.34G>A 0% COSM517 12 25245351
26 SNV KRAS G12R c.34G>C 0% COSM518 12 25245351
27 SNV KRAS G12A c.35G>C 0% COSM522 12 25245350
  • As a negative control of Onco SNV gDNA Reference Standards
  • Other allele mutation frequencies obtained by mixing with Onco SNV gDNA series Reference Standard
  • In combination with the use of Onco SNV gDNA series Reference Standard, different mutation frequency gradients are set to verify the accuracy of the process
IB-GW-OGTM005-Package Insert： download
IB-GW-OGTM005-Flyer： download
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