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Onco Wildtype gDNA Reference Standard

CatalogNo. specification inventory
IB-GW-OGTM005 5µg/Tube 9999
  • Intro
Next generation sequencing (NGS) platforms give researchers much greater power when profiling the complex human genome. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers.           
Genewell’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels.          
Onco Wildtype gDNA Reference Standard is mainly used as negative controls used to assess the performance of assays that detect somatic mutations,such as NGS and qPCR etc. It matches Onco Structural Multiplex 5% gDNA Reference Standard, Onco SNV Multiplex 5% gDNA  Reference Standard,Onco SNV Multiplex 1-25% gDNA  Reference Standard etc. to  to evaluate specificity and sensitivity of assays.
  • Product information
  • Application fields
  • File download
浓度 30ng/µL
保存溶剂 Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
存储温度 2-8˚C
保质期 36 months
规格 5µg/tube

Authenticated Mutation

Test Test Method Acceptance Criteria
Allele Frequency (AF) and Genotype Droplet Digital PCR AF = 0%, accepted range =≤0.1%; AF ＜5%, accepted range =± 30%; 5% ≤AF ＜ 10%, accepted range = ± 20%; 10% ≤AF, accepted range = ± 10%; Copy Number＜ 5, accepted range =± 30%; 5 ≤ Copy Number ＜10, accepted range = ± 20%; 10 ≤ Copy Number, accepted range = ± 10%
Quality Agarose gel electrophoresis Bright band of high molecular weight
Quantification Qubit? dsDNA BR Assay 30 ng/μL±10%
Number Variant Type Mutation CDS Mutation Expected Allelic Frequency(%)/Copy Number COSMIC ID Chromosome Number Genomosome Position
1 SNV AKT1 E17K c.49G＞A 0% COSM33765 14 104780214
2 SNV BRAF V600E c.1799T＞A 0% COSM476 7 140753336
3 SNV EGFR G719S c.2155G＞A 0% COSM6252 7 55174014
4 SNV EGFR L858R c.2573T＞G 0% COSM6224 7 55191822
5 SNV EGFR T790M c.2369C＞T 0% COSM6240 7 55181378
6 Insertion EGFR V769_D770insASV c.2307_2308insGCCAGCGTG 0% COSM12376 7 55181317-55181318
7 Deletion EGFR ΔE746_A750 c.2235_2249del15 0% COSM6223 7 55174772-55174786
8 Deletion FLT3 ΔI836 c.2506_2508delATC 0% COSM797 13 28018500-28018502
9 SNV KIT D816V c.2447A＞T 0% COSM1314 4 54733155
10 ANV KRAS A146T c.436G＞A 0% COSM19404 12 25225628
11 SNV KRAS G12D c.35G＞A 0% COSM521 12 25245350
12 SNV KRAS G13D c.38G＞A 0% COSM532 12 25245347
13 SNV NRAS Q61K c.181C＞A 0% COSM580 1 114713909
14 SNV PIK3CA E545K c.1633G＞A 0% COSM763 3 179218303
15 SNV PIK3CA H1047R c.3140A＞G 0% COSM775 3 179234297
16 Fusion CD74-ROS1 Fusion NA 0% COSF1200 6,5 117324415,150404680
17 Fusion EML4-ALK Fusion NA 0% COSF474 2,2 42264951,29223528
18 CNV ERBB2 Amplification NA 2 copies NA 17 39687914-39730426
19 CNV MET Amplification NA 2 copies NA 7 116672196-116798386
20 Fusion EML4-ALK Fusion V1 NA 0% COSF408 2,2 4229551629223520
21 SNV KRAS G12C c.34G>T 0% COSM516 12 25245351
22 SNV KRAS G12V c.35G>T 0% COSM520 12 25245350
23 SNV EGFR L861Q c.2582T>A 0% COSM6213 7 55191831
24 SNV EGFR S768I c.2303G>T 0% COSM6241 7 55181312
25 SNV KRAS G12S c.34G>A 0% COSM517 12 25245351
26 SNV KRAS G12R c.34G>C 0% COSM518 12 25245351
27 SNV KRAS G12A c.35G>C 0% COSM522 12 25245350
  • As a negative control of Onco SNV gDNA Reference Standards
  • Other allele mutation frequencies obtained by mixing with Onco SNV gDNA series Reference Standard
  • In combination with the use of Onco SNV gDNA series Reference Standard, different mutation frequency gradients are set to verify the accuracy of the process
IB-GW-OGTM005-product manual-EN-20220601： download
IB-GW-OGTM005-product insert-20220601： download
  • Similar Product
CatalogNo：
IB-GW-OCTM005
Format：
Liquid Biopsy(ctDNA)